Composite

Part:BBa_K4616012

Designed by: Haoyu Zhang   Group: iGEM23_BIT-China   (2023-10-10)


Fluorescent protein detection gene circuitry in E.coli

Usage

This is one of the E.coli fluorescent protein detection gene circuits in the BIT-China 2023 IGEM project "Light's control iT".

igem23-part-pblrep-rbs34-mcherry.png

Figure 1. E.coli transformation experimental verification result. M-marker. 1-PBLrep_rbs34_mCherry (composite part K4616012,765 bp).

Biology

When el222 gene is expressed,the photosensitive transcription factor EL222 is generated. Without 450 nm light illumination, the photosensitive transcription factor EL222 stop dimerization and then the downstream gene PBLrep functions as a promoter, then the transcript of gene mCherry starts. mCherry is a red fluorescent protein from mushroom coral, which is a various of essential reporter in cell and molecular biology.

igem23-part-ecoli-rfp1.png

Figure 2.Light control system in E.coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 760
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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